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Genome Res. 2012 Jun;22(6):1139-43. doi: 10.1101/gr.136242.111. Epub 2012 Mar 30.

Ultra-low-input, tagmentation-based whole-genome bisulfite sequencing.

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  • 1Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA.

Abstract

We have adapted transposase-based in vitro shotgun library construction ("tagmentation") for whole-genome bisulfite sequencing. This method, Tn5mC-seq, enables a >100-fold reduction in starting material relative to conventional protocols, such that we generate highly complex bisulfite sequencing libraries from as little as 10 ng of input DNA, and ample useful sequences from 1 ng of input DNA. We demonstrate Tn5mC-seq by sequencing the methylome of a human lymphoblastoid cell line to ∼8.6× high-quality coverage of each strand.

PMID:
22466172
[PubMed - indexed for MEDLINE]
PMCID:
PMC3371708
Free PMC Article
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