Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Nanomedicine. 2013 Jan;9(1):130-40. doi: 10.1016/j.nano.2012.03.004. Epub 2012 Mar 28.

Nanoliposomal minocycline for ocular drug delivery.

Author information

  • 1Department of Pharmacology, Pennsylvania State College of Medicine, Hummelstown, PA 17036, USA.

Abstract

Nanoliposomal technology is a promising drug delivery system that could be employed to improve the pharmacokinetic properties of clearance and distribution in ocular drug delivery to the retina. We developed a nanoscale version of an anionic, cholesterol-fusing liposome that can encapsulate therapeutic levels of minocycline capable of drug delivery. We demonstrate that size extrusion followed by size-exclusion chromatography can form a stable 80-nm liposome that encapsulates minocycline at a concentration of 450 ± 30 μM, which is 2% to 3% of loading material. More importantly, these nontoxic nanoliposomes can then deliver 40% of encapsulated minocycline to the retina after a subconjunctival injection in the STZ model of diabetes. Efficacy of therapeutic drug delivery was assessed via transcriptomic and proteomic biomarker panels. For both the free minocycline and encapsulated minocycline treatments, proinflammatory markers of diabetes were downregulated at both the messenger RNA and protein levels, validating the utility of biomarker panels for the assessment of ocular drug delivery vehicles.

FROM THE CLINICAL EDITOR:

Authors developed a nano-liposome that can encapsulate minocycline for optimized intraocular drug delivery. These nontoxic nanoliposomes delivered 40% of encapsulated minocycline to the retina after a subconjunctival injection in a diabetes model.

Copyright © 2013 Elsevier Inc. All rights reserved.

PMID:
22465498
[PubMed - indexed for MEDLINE]
PMCID:
PMC3762261
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Write to the Help Desk