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J Microbiol Methods. 2012 Jun;89(3):174-8. doi: 10.1016/j.mimet.2012.03.012. Epub 2012 Mar 23.

High-throughput species identification of enterococci using pyrosequencing.

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  • 1Agriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge, Alberta, Canada T1J 4B1.


Here we report the development and validation of an automated high-throughput pyrosequencing-based method for the reliable identification of isolated Enterococcus species. This method exploits the discrete species-specificity of hypervariable groES-EL spacer region and utilizes a universal dispensation order optimized for a wide range of Enterococcus species.

Crown Copyright © 2012. Published by Elsevier B.V. All rights reserved.

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