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Pennsylvania Muscle Institute and Department of Physiology, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, USA.
Recordings from single molecule experiments can be aggregated to determine average kinetic properties of the system under observation. The kinetics after a synchronized reaction step can be interpreted using all of the standard tools developed for ensemble perturbation experiments. The kinetics leading up to a synchronized event, determined by the lifetimes of the preceding states; however, are not as obvious if the reaction has reversible steps or branches. Here we describe a general procedure for dealing with these situations.
Copyright © 2012 Biophysical Society. Published by Elsevier Inc. All rights reserved.
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