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Dev Biol. 2012 May 15;365(2):384-94. doi: 10.1016/j.ydbio.2012.03.005. Epub 2012 Mar 14.

Drosophila Argonaute 1 and its miRNA biogenesis partners are required for oocyte formation and germline cell division.

Author information

  • 1MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, South Parks Road, University of Oxford, Oxford, UK.

Abstract

Argonaute 1 (Ago1) is a member of the Argonaute/PIWI protein family involved in small RNA-mediated gene regulation. In Drosophila, Ago1 plays a specific role in microRNA (miRNA) biogenesis and function. Previous studies have demonstrated that Ago1 regulates the fate of germline stem cells. However, the function of Ago1 in other aspects of oogenesis is still elusive. Here we report the function of Ago1 in developing egg chambers. We find that Ago1 protein is enriched in the oocytes and is also highly expressed in the cytoplasm of follicle cells. Clonal analysis of multiple ago1 mutant alleles shows that many mutant egg chambers contain only 8 nurse cells without an oocyte which is phenocopied in dicer-1, pasha and drosha mutants. Our results suggest that Ago1 and its miRNA biogenesis partners play a role in oocyte determination and germline cell division in Drosophila.

Crown Copyright © 2012. Published by Elsevier Inc. All rights reserved.

PMID:
22445511
[PubMed - indexed for MEDLINE]
PMCID:
PMC3763516
Free PMC Article

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