Format

Send to:

Choose Destination
See comment in PubMed Commons below
Mol Cell. 2012 Apr 27;46(2):159-70. doi: 10.1016/j.molcel.2012.02.013. Epub 2012 Mar 22.

Embryonic stem cells induce pluripotency in somatic cell fusion through biphasic reprogramming.

Author information

  • 1Department of Human Genetics, University of Chicago, Howard Hughes Medical Institute, Chicago, IL 60637, USA.

Abstract

It is a long-held paradigm that cell fusion reprograms gene expression but the extent of reprogramming and whether it is affected by the cell types employed remain unknown. We recently showed that the silencing of somatic genes is attributable to either trans-acting cellular environment or cis-acting chromatin context. Here, we examine how trans- versus cis-silenced genes in a somatic cell type behave in fusions to another somatic cell type or to embryonic stem cells (ESCs). We demonstrate that while reprogramming of trans-silenced somatic genes occurs in both cases, reprogramming of cis-silenced somatic genes occurs only in somatic-ESC fusions. Importantly, ESCs reprogram the somatic genome in two distinct phases: trans-reprogramming occurs rapidly, independent of DNA replication, whereas cis-reprogramming occurs with slow kinetics requiring DNA replication. We also show that pluripotency genes Oct4 and Nanog are cis-silenced in somatic cells. We conclude that cis-reprogramming capacity is a fundamental feature distinguishing ESCs from somatic cells.

Copyright © 2012 Elsevier Inc. All rights reserved.

PMID:
22445485
[PubMed - indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk