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Chembiochem. 2012 May 7;13(7):959-63. doi: 10.1002/cbic.201200045. Epub 2012 Mar 20.

A universal expression tag for structural and functional studies of proteins.

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  • 1Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Strasse 9, 60438 Frankfurt, Germany.


Modified ubiquitin sequences, each completed with a His tag and a TEV cleavage site, were designed to enhance the expression of protein/peptide targets. With this new system we have been able to characterize several peptide-protein interactions by ITC and by NMR and CD spectroscopic methods, including the interactions of LIR domains with autophagy modifiers.

Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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