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PLoS One. 2012;7(3):e32671. doi: 10.1371/journal.pone.0032671. Epub 2012 Mar 9.

Toxoplasma gondii chromodomain protein 1 binds to heterochromatin and colocalises with centromeres and telomeres at the nuclear periphery.

Author information

  • 1Center for Infection and Immunity of Lillle, CNRS UMR 8204, INSERM U 1019, Université Lille Nord de France, Institut Pasteur de Lille, Lille, France. Stan.Tomavo@pasteur-lille.fr

Abstract

BACKGROUND:

Apicomplexan parasites are responsible for some of the most deadly parasitic diseases afflicting humans, including malaria and toxoplasmosis. These obligate intracellular parasites exhibit a complex life cycle and a coordinated cell cycle-dependant expression program. Their cell division is a coordinated multistep process. How this complex mechanism is organised remains poorly understood.

METHODS AND FINDINGS:

In this study, we provide evidence for a link between heterochromatin, cell division and the compartmentalisation of the nucleus in Toxoplasma gondii. We characterised a T. gondii chromodomain containing protein (named TgChromo1) that specifically binds to heterochromatin. Using ChIP-on-chip on a genome-wide scale, we report TgChromo1 enrichment at the peri-centromeric chromatin. In addition, we demonstrate that TgChromo1 is cell-cycle regulated and co-localised with markers of the centrocone. Through the loci-specific FISH technique for T. gondii, we confirmed that TgChromo1 occupies the same nuclear localisation as the peri-centromeric sequences.

CONCLUSION:

We propose that TgChromo1 may play a role in the sequestration of chromosomes at the nuclear periphery and in the process of T. gondii cell division.

PMID:
22427862
[PubMed - indexed for MEDLINE]
PMCID:
PMC3302879
Free PMC Article

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