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Arch Med Sci. 2010 Dec;6(6):964-70. doi: 10.5114/aoms.2010.19309. Epub 2010 Dec 29.

Interleukin-18 expression and the response to treatment in patients with psoriasis.

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  • 1Clinical and Chemical Pathology Department, National Research Center, Cairo, Egypt.

Abstract

INTRODUCTION:

The aim of the study was to demonstrate Interleukin-18 (IL-18) expression in keratinocytes from psoriatic lesions in comparison to keratinocytes from uninvolved skin and to study the change of expression after therapeutic interventions.

MATERIAL AND METHODS:

This study included 16 patients of different clinical subtypes of psoriasis. IL-18 gene expression analysis was performed using real-time quantitative PCR. Three biopsies were obtained from each patient. Two were taken from the lesional psoriatic skin and from uninvolved skin before starting treatment. A third lesional skin biopsy was taken at the end of two months' treatment course. The treatment was in the form of topical steroids or oral systemic methotrexate.

RESULTS:

Of all 16 studied patients significantly increased IL-18 expression was noted in keratinocytes from psoriatic lesions before and after treatment when compared to keratinocytes from uninvolved skin (P = 0.001 and 0.002 respectively). The IL-18 expression in the skin lesions after treatment was significantly lower than lesional skin before treatment (P = 0.023). In psoriatic skin lesions of all studied patients IL-18 expression was significantly correlated with disease duration (r = 0.40 and P = 0.01) and clinical severity of psoriasis (r = 0.72 and P = 0.001).

CONCLUSIONS:

Increased IL-18 expression in keratinocytes from psoriatic lesions of our patients and its correlation with disease duration and severity supported the concept which views psoriasis as a T-cell-mediated autoimmune disease. This could establish therapeutic and preventive approaches for psoriasis that ultimately lead to improved outcomes for patients.

KEYWORDS:

effect of therapy; interleukins; psoriasis; real-time PCR

PMID:
22427774
[PubMed]
PMCID:
PMC3302712
Free PMC Article
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