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Nanomedicine (Lond). 2012 Jun;7(6):867-76. doi: 10.2217/nnm.11.187. Epub 2012 Mar 15.

Induction of immune tolerance in mice with a novel mucosal nanoemulsion adjuvant and self-antigen.

Author information

  • 1Michigan Nanotechnology Institute for Medicine & Biological Sciences, Department of Internal Medicine, University of Michigan, Ann Arbor, MI, USA. shidasui@med.umich.edu

Abstract

AIM:

The aim of this study was to investigate the impact of a novel nanoemulsion (NE) adjuvant, a soybean oil emulsion, on autoimmune response. To this end, we used murine thyroglobulin (mTg)-induced experimental autoimmune thyroiditis in mice as a study model.

MATERIALS & METHODS:

Mice received NE or NE + mTg by nasal delivery. At 1 week after the second nasal delivery of NE with or without mTg, all mice were immunized with mTg and lipopolysaccharides to induce experimental autoimmune thyroiditis.

RESULTS:

Compared with controls, mTg-NE-treated mice had much more antigens accumulated in the nasal passage and thymus and developed a milder form of thyroiditis. This was accompanied by an increase in IL-10, IL-17 and reduced IFN-γ. The production of anti-mTg antibodies was significantly decreased in mTg-NE-treated mice. The percentage of Tregs in cervical lymph nodes was higher in mTg-NE-treated mice than NE-treated mice. Furthermore, Foxp3 and TGF-β levels were prominently enhanced in mTg-NE-treated mice.

CONCLUSION:

This study indicates that a low dose of mTg in NE can significantly enhance antigen uptake and Tregs, resulting in inhibition of experimental autoimmune thyroiditis development.

PMID:
22420425
[PubMed - indexed for MEDLINE]
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