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Anal Chem. 2012 Apr 17;84(8):3811-9. doi: 10.1021/ac300532z. Epub 2012 Apr 2.

Nontargeted nucleotide analysis based on benzoylhistamine labeling-MALDI-TOF/TOF-MS: discovery of putative 6-oxo-thymine in DNA.

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  • 1Pharmaceutical Science and Barnett Institute, Northeastern University, Boston, Massachusetts 02115, United States.

Abstract

A method for nontargeted analysis of modified nucleotides in DNA (and RNA) is reported based on labeling with benzoylhistamine (BH) followed by MALDI-MS. The method provides deoxynucleotide-specific detection, accurate measurement of molecular ions, high sensitivity, semiquantitation, and, to the extent studied to date, normalization of response within a factor of <3.

PMID:
22409256
[PubMed - indexed for MEDLINE]
PMCID:
PMC3341171
Free PMC Article
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