Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Science. 2012 Mar 9;335(6073):1194. doi: 10.1126/science.1218298.

Fluorescence imaging of cellular metabolites with RNA.

Author information

  • 1Department of Pharmacology, Weill Medical College, Cornell University, New York, NY 10065, USA.

Abstract

Genetically encoded sensors are powerful tools for imaging intracellular metabolites and signaling molecules. However, developing sensors is challenging because they require proteins that undergo conformational changes upon binding the desired target molecule. We describe an approach for generating fluorescent sensors based on Spinach, an RNA sequence that binds and activates the fluorescence of a small-molecule fluorophore. We show that these sensors can detect a variety of different small molecules in vitro and in living cells. These RNAs constitute a versatile approach for fluorescence imaging of small molecules and have the potential to detect essentially any cellular biomolecule.

PMID:
22403384
[PubMed - indexed for MEDLINE]
PMCID:
PMC3303607
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk