Aim: To prepare high-performance and specific monoclonal antibody (mAb) against Vibrio vulnificus and carry out characterization.
Methods: BALB/c mice were immunized with Vibrio vulnificus protein, and hybridoma cells against Vibrio vulnificus were produced by cellsion technique. The titers of mAbs against vvhA and cross-reaction between the anti-vvhA mAb and other other important marine bacteria were screened by ELISA and Western blot.
Results: Five strains of hybridoma were obtained. Identification result indicated that 5 mAbs have favourable specifity and immunoreactivity.
Conclusion: Specific mAbs against Vibrio vulnificus were produced which provides an important preparation for establishing rapid-detection kit of detecting Vibrio vulnificus.