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World J Gastroenterol. 2012 Feb 28;18(8):785-93. doi: 10.3748/wjg.v18.i8.785.

Vaccination with dendritic cells pulsed with hepatitis C pseudo particles induces specific immune responses in mice.

Author information

  • 1Department of Gastroenterology and Hepatology, University Hospital Heidelberg, D-69120 Heidelberg, Germany. kilian_weigand@med.uni-heidelberg.de

Abstract

AIM:

To explore dendritic cells (DCs) multiple functions in immune modulation.

METHODS:

We used bone-marrow derived dendritic cells from BALB/c mice pulsed with pseudo particles from the hepatitis C virus to vaccinate naive BALB/c mice. Hepatitis C virus (HCV) pseudo particles consist of the genotype 1b derived envelope proteins E1 and E2, covering a non-HCV core structure. Thus, not a single epitope, but the whole "viral surface" induces immunogenicity. For vaccination, mature and activated DC were injected subcutaneously twice.

RESULTS:

Humoral and cellular immune responses measured by enzyme-linked immunosorbent assay and interferon-gamma enzyme-linked immunosorbent spot test showed antibody production as well as T-cells directed against HCV. Furthermore, T-cell responses confirmed two highly immunogenic regions in E1 and E2 outside the hypervariable region 1.

CONCLUSION:

Our results indicate dendritic cells as a promising vaccination model for HCV infection that should be evaluated further.

KEYWORDS:

Dendritic cell; Hepatitis C; Immune responses; Pseudo particles; Vaccination

PMID:
22371638
[PubMed - indexed for MEDLINE]
PMCID:
PMC3286141
Free PMC Article
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