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EMBO Rep. 2012 Apr;13(4):331-8. doi: 10.1038/embor.2012.18.

Cargo ubiquitination is essential for multivesicular body intralumenal vesicle formation.

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  • 1Molecular Physiology and Biophysics, University of Iowa, Iowa City, Iowa 52246, USA.


The efficient formation of a variety of transport vesicles is influenced by the presence of cargo, suggesting that cargo itself might have a defining role in vesicle biogenesis. However, definitive in vivo experiments supporting this concept are lacking, as it is difficult to eliminate endogenous cargo. The Endosomal Sorting Complexes Required for Transport (ESCRT) apparatus sorts ubiquitinated membrane proteins into endosomal intralumenal vesicles (ILVs) that accumulate within multivesicular bodies. Here we show that cargo ubiquitination is required for effective recruitment of the ESCRT machinery onto endosomal membranes and for the subsequent formation of ILVs.

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