Display Settings:

Format

Send to:

Choose Destination
Am J Physiol Lung Cell Mol Physiol. 2012 May 15;302(10):L1098-106. doi: 10.1152/ajplung.00253.2011. Epub 2012 Feb 24.

Chloride secretion by cultures of pig tracheal gland cells.

Author information

  • 1Department of Physiology and Membrane Biology, University of California-Davis, CA 95616-8664, USA. jhwiddicombe@ucdavis.edu

Abstract

Malfunction of airway submucosal glands contributes to the pathology of cystic fibrosis (CF), and cell cultures of CF human airway glands show defects in Cl(-) and water transport. Recently, a transgenic pig model of CF (the CF pig) has been developed. Accordingly, we have developed cell cultures of pig airway gland epithelium for use in investigating alterations in gland function in CF. Our cultures form tight junctions (as evidenced by high transepithelial electrical resistance) and show high levels of active anion secretion (measured as amiloride-insensitive short-circuit current). In agreement with recent results on human airway glands, neurohumoral agents that elevate intracellular Ca(2+) potently stimulated anion secretion, while elevation of cAMP was comparatively ineffective. Our cultures express lactoferrin and lysozyme (serous gland cell markers) and MUC5B (the main mucin of airway glands). They are, therefore, potentially useful in determining if CF-related alterations in anion transport result in altered secretion of serous cell antimicrobial agents or mucus.

PMID:
22367783
[PubMed - indexed for MEDLINE]
PMCID:
PMC3362257
Free PMC Article

Images from this publication.See all images (5)Free text

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.
Fig. 5.
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk