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Mol Cell Proteomics. 2012 Jul;11(7):M111.014746. doi: 10.1074/mcp.M111.014746. Epub 2012 Feb 23.

Targeted proteomics of the eicosanoid biosynthetic pathway completes an integrated genomics-proteomics-metabolomics picture of cellular metabolism.

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  • 1Department of Biology, Institute of Molecular Systems Biology and Competence Center for Systems Physiology and Metabolic Disease, ETH Zurich, 8093 Zurich, Switzerland.

Abstract

Eicosanoids constitute a diverse class of bioactive lipid mediators that are produced from arachidonic acid and play critical roles in cell signaling and inflammatory aspects of numerous diseases. We have previously quantified eicosanoid metabolite production in RAW264.7 macrophage cells in response to Toll-like receptor 4 signaling and analyzed the levels of transcripts coding for the enzymes involved in the eicosanoid metabolite biosynthetic pathways. We now report the quantification of changes in protein levels under similar experimental conditions in RAW264.7 macrophages by multiple reaction monitoring mass spectrometry, an accurate targeted protein quantification method. The data complete the first fully integrated genomic, proteomic, and metabolomic analysis of the eicosanoid biochemical pathway.

PMID:
22361236
[PubMed - indexed for MEDLINE]
PMCID:
PMC3394947
Free PMC Article

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