Format

Send to:

Choose Destination
See comment in PubMed Commons below
J Microsc. 2012 May;246(2):113-23. doi: 10.1111/j.1365-2818.2012.03596.x. Epub 2012 Feb 22.

Quantitative comparison between full-spectrum and filter-based imaging in hyperspectral fluorescence microscopy.

Author information

  • 1Department of Bioengineering, Rice University, 6100 Main Street, Houston, TX 77005, USA.

Abstract

We implement a filterless illumination scheme on a hyperspectral fluorescence microscope to achieve full-range spectral imaging. The microscope employs polarisation filtering, spatial filtering and spectral unmixing filtering to replace the role of traditional filters. Quantitative comparisons between full-spectrum and filter-based microscopy are provided in the context of signal dynamic range and accuracy of measured fluorophores' emission spectra. To show potential applications, a five-colour cell immunofluorescence imaging experiment is theoretically simulated. Simulation results indicate that the use of proposed full-spectrum imaging technique may result in three times improvement in signal dynamic range compared to that can be achieved in the filter-based imaging.

© 2012 The Authors Journal of Microscopy © 2012 Royal Microscopical Society.

PMID:
22356127
[PubMed - indexed for MEDLINE]
PMCID:
PMC3323773
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Wiley Icon for PubMed Central
    Loading ...
    Write to the Help Desk