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Oncol Rep. 2012 May;27(5):1587-91. doi: 10.3892/or.2012.1685. Epub 2012 Feb 13.

SNAI1 overexpression induces stemness and promotes ovarian cancer cell invasion and metastasis.

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  • 1Center for Reproductive Medicine, Tianjin Central Hospital of Obstetrics and Gynecology, Tianjin Medical University, Tianjin 300100, PR China.

Abstract

Ovarian cancer is the fifth most common cancer among women worldwide. Detection of metastasis of ovarian cancer is crucial for diagnosis and prolongs the life of patients. This study focused on whether SNAI1 overexpression relates to invasion of ovarian cancer in vitro and in vivo. Invasion, colony formation and wound healing assays and flow cytometric analysis were performed to test the invasion and proliferation of SKOV3 ovarian cancer cells after transfection. The effect of SNAI1 on ovarian cancer in vivo was validated using a murine xenograft model. In vitro, SNAI1 upregulation led to an increased percent of CD133+ SKOV3 cells and promoted SKOV3 cell invasion and proliferation. In vivo, the SNAI1 overexpression group showed the highest rate of tumor growth compared with SNAI2 and the control group (60 and 50%, respectively). Our results show that SNAI1 expression induces an increase in the number of CD133+ cells, a change important for the epithelial to mesenchymal transition and the proliferation in ovarian cancer. It is suggested that SNAI1 may serve as a novel target for ovarian cancer prediction and therapy.

PMID:
22344746
[PubMed - indexed for MEDLINE]
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