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Biophys J. 2012 Jan 18;102(2):L5-7. doi: 10.1016/j.bpj.2011.12.011.

Mechanosensing in T lymphocyte activation.

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  • 1Department of Biomedical Engineering, Columbia University, New York, New York, USA.

Abstract

Mechanical forces play an increasingly recognized role in modulating cell function. This report demonstrates mechanosensing by T cells, using polyacrylamide gels presenting ligands to CD3 and CD28. Naive CD4 T cells exhibited stronger activation, as measured by attachment and secretion of IL-2, with increasing substrate elastic modulus over the range of 10-200 kPa. By presenting these ligands on different surfaces, this report further demonstrates that mechanosensing is more strongly associated with CD3 rather than CD28 signaling. Finally, phospho-specific staining for Zap70 and Src family kinase proteins suggests that sensing of substrate rigidity occurs at least in part by processes downstream of T-cell receptor activation. The ability of T cells to quantitatively respond to substrate rigidly provides an intriguing new model for mechanobiology.

Copyright © 2012 Biophysical Society. Published by Elsevier Inc. All rights reserved.

PMID:
22339876
[PubMed - indexed for MEDLINE]
PMCID:
PMC3260692
Free PMC Article

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