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J Am Chem Soc. 2012 Mar 14;134(10):4489-92. doi: 10.1021/ja211312u. Epub 2012 Feb 29.

Chemoenzymatic probes for detecting and imaging fucose-α(1-2)-galactose glycan biomarkers.

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  • 1Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 East California Boulevard, Pasadena, California 91125, United States.

Abstract

The disaccharide motif fucose-α(1-2)-galactose (Fucα(1-2)Gal) is involved in many important physiological processes, such as learning and memory, inflammation, asthma, and tumorigenesis. However, the size and structural complexity of Fucα(1-2)Gal-containing glycans have posed a significant challenge to their detection. We report a new chemoenzymatic strategy for the rapid, sensitive detection of Fucα(1-2)Gal glycans. We demonstrate that the approach is highly selective for the Fucα(1-2)Gal motif, detects a variety of complex glycans and glycoproteins, and can be used to profile the relative abundance of the motif on live cells, discriminating malignant from normal cells. This approach represents a new potential strategy for biomarker detection and expands the technologies available for understanding the roles of this important class of carbohydrates in physiology and disease.

PMID:
22339094
[PubMed - indexed for MEDLINE]
PMCID:
PMC3303202
Free PMC Article
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