Objective: To investigate the mechanical stress of four different mechanical bioreactors on the proliferation of in vitro human bladder smooth muscle cells (hBSMCs) and to design a feasible stress applying device.
Methods: We designed four mechanical bioreactors successively: Pressure Circular Tank, Strain Tank, Water Column and Pressure Culture Flask. hBSMCs were cultured under 40 cm H2O sustained hydrostatic pressure or 7.7 N-strian (pressure test group), and the controls were cultured without pressure. Cell survival and proliferation status of hBSMCs after the applying of mechanical stress were evaluated by the using of CCK8 assay, immunofluorescence staining cytoskeleton alpha-actin and RT-PCR detected PCNA mRNA.
Results: There were no significance differences of CCK8 assay, immunofluorescence staining cytoskeleton alpha-actin and RT-PCR detected PCNA mRNA between pressure group and control group (P>0.05), and also no differences among the four pressure groups (P>0.05).
Conclusion: The four mechanical bioreactors are no toxic to in vitro human bladder smooth muscle cells. The 40 cmH2O hydrostatic pressure or 7.7 N-strian can not induce the proliferation of hBSMC.