Three new iridoids, valeriandoids D-F (1-3), have been isolated from the roots of Valeriana jatamansi. Structure elucidation, especially the positions of the acyloxy groups, and complete (1)H- and (13)C-NMR assignments of these new compounds were carried out using one- and two-dimensional NMR measurements, including (1)H- and (13)C-NMR, DEPT-135, (1)H-(1)H COSY, HMQC, HMBC, and HR-ESI-MS experiments.