Inactivation of recombinant bacteriophage lambda by use of chemical agents and UV radiation

Appl Environ Microbiol. 2012 Apr;78(8):3033-6. doi: 10.1128/AEM.06800-11. Epub 2012 Feb 10.

Abstract

Several approaches for the inactivation of bacteriophage lambda, including UV germicidal irradiation (UVGI) and the chemical agents Virkon-S, Chloros, Decon-90, and sodium hydroxide (NaOH), were compared. Virkon, NaOH, and UVGI caused a ≥7-log(10) reduction in phage titers. This study successfully describes several methods with potential for bacteriophage inactivation in industrial settings.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antiviral Agents / metabolism*
  • Bacteriophage lambda / drug effects
  • Bacteriophage lambda / genetics
  • Bacteriophage lambda / physiology*
  • Bacteriophage lambda / radiation effects
  • Microbial Viability / drug effects*
  • Microbial Viability / radiation effects*
  • Peroxides
  • Sodium Compounds
  • Sulfuric Acids
  • Ultraviolet Rays*
  • Viral Load
  • Virus Inactivation*

Substances

  • Antiviral Agents
  • Peroxides
  • Sodium Compounds
  • Sulfuric Acids
  • sodium hydride
  • monoperoxysulfate