Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Curr Biol. 2012 Mar 6;22(5):414-9. doi: 10.1016/j.cub.2012.01.031. Epub 2012 Feb 9.

Proteomic analysis of mammalian primary cilia.

Author information

  • 1Department of Biochemistry & Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA.

Abstract

The primary cilium is a microtubule-based organelle that senses extracellular signals as a cellular antenna. Primary cilia are found on many types of cells in our body and play important roles in development and physiology. Defects of primary cilia cause a broad class of human genetic diseases called ciliopathies. To gain new insights into ciliary functions and better understand the molecular mechanisms underlying ciliopathies, it is of high importance to generate a catalog of primary cilia proteins. In this study, we isolated primary cilia from mouse kidney cells by using a calcium-shock method and identified 195 candidate primary cilia proteins by MudPIT (multidimensional protein identification technology), protein correlation profiling, and subtractive proteomic analysis. Based on comparisons with other proteomic studies of cilia, around 75% of our candidate primary cilia proteins are shared components with motile or specialized sensory cilia. The remaining 25% of the candidate proteins are possible primary cilia-specific proteins. These possible primary cilia-specific proteins include EVC2, INPP5E, and inversin, several of which have been linked to known ciliopathies. We have performed the first reported proteomic analysis of primary cilia from mammalian cells. These results provide new insights into primary cilia structure and function.

Copyright © 2012 Elsevier Ltd. All rights reserved.

PMID:
22326026
[PubMed - indexed for MEDLINE]
PMCID:
PMC3298568
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for PubMed Central
    Loading ...
    Write to the Help Desk