J Med Primatol. 1990;19(3-4):421-9.

Nef genes of SIV.

Kestler HW 3rd, Mori K, Silva DP, Kodama T, King NW, Daniel MD, Desrosiers RC.

Source

New England Regional Primate Research Center, Southborough, MA.

Abstract

Molecular clones of SIVmac were constructed that differed only in sequences within the nef gene. DEAE-transfection of viral DNA containing an open from of nef yielded virus that replicated with similar kinetics and to a similar extent in macaque peripheral blood lymphocyte (PBL) cultures as virus with a deletion or stop codon within nef. Rhesus monkeys that received each kind of molecularly cloned virus became infected. Our results additionally suggest that mutant forms of virus are selected in vitro while open, functional forms are selected in vivo. In animals infected with virus containing a stop codon within nef, reversion of the stop codon to a coding codon was demonstrated in five of five clones analyzed. These results indicate that nef is playing some role crucial to the virus life cycle in vivo.

PMID:: 2231693; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

MeSH Terms

Substances

Codon

Grant Support

Supplemental Content

Save items

Related citations in PubMed

Importance of the nef gene for maintenance of high virus loads and for development of AIDS. [Cell. 1991]
Importance of the nef gene for maintenance of high virus loads and for development of AIDS.
Kestler HW 3rd, Ringler DJ, Mori K, Panicali DL, Sehgal PK, Daniel MD, Desrosiers RC. Cell. 1991 May 17; 65(4):651-62.
Molecular and biological characterization of simian immunodeficiency virus macaque strain 32H proviral clones containing nef size variants. [J Gen Virol. 1994]
Molecular and biological characterization of simian immunodeficiency virus macaque strain 32H proviral clones containing nef size variants.
Rud EW, Cranage M, Yon J, Quirk J, Ogilvie L, Cook N, Webster S, Dennis M, Clarke BE. J Gen Virol. 1994 Mar; 75 ( Pt 3):529-43.
Importance of vpr for infection of rhesus monkeys with simian immunodeficiency virus. [J Virol. 1993]
Importance of vpr for infection of rhesus monkeys with simian immunodeficiency virus.
Lang SM, Weeger M, Stahl-Hennig C, Coulibaly C, Hunsmann G, Müller J, Müller-Hermelink H, Fuchs D, Wachter H, Daniel MM. J Virol. 1993 Feb; 67(2):902-12.
The role of upstream U3 sequences in the pathogenesis of simian immunodeficiency virus-induced AIDS in rhesus monkeys. [J Virol. 1994]
The role of upstream U3 sequences in the pathogenesis of simian immunodeficiency virus-induced AIDS in rhesus monkeys.
Ilyinskii PO, Daniel MD, Simon MA, Lackner AA, Desrosiers RC. J Virol. 1994 Sep; 68(9):5933-44.
The nef gene of simian immunodeficiency virus SIVmac1A11. [J Virol. 1992]
The nef gene of simian immunodeficiency virus SIVmac1A11.
Unger RE, Marthas ML, Pratt-Lowe E, Padrid PA, Luciw PA. J Virol. 1992 Sep; 66(9):5432-42.

See reviews... See all...

Cited by 9 PubMed Central articles

Circulating Nef induces dyslipidemia in simian immunodeficiency virus-infected macaques by suppressing cholesterol efflux. [J Infect Dis. 2010]
Circulating Nef induces dyslipidemia in simian immunodeficiency virus-infected macaques by suppressing cholesterol efflux.
Asztalos BF, Mujawar Z, Morrow MP, Grant A, Pushkarsky T, Wanke C, Shannon R, Geyer M, Kirchhoff F, Sviridov D, et al. J Infect Dis. 2010 Aug 15; 202(4):614-23.
Selective downregulation of rhesus macaque and sooty mangabey major histocompatibility complex class I molecules by Nef alleles of simian immunodeficiency virus and human immunodeficiency virus type 2. [J Virol. 2008]
Selective downregulation of rhesus macaque and sooty mangabey major histocompatibility complex class I molecules by Nef alleles of simian immunodeficiency virus and human immunodeficiency virus type 2.
DeGottardi MQ, Specht A, Metcalf B, Kaur A, Kirchhoff F, Evans DT. J Virol. 2008 Mar; 82(6):3139-46. Epub 2008 Jan 16.
Entire genome of a strain of human immunodeficiency virus type 1 with a deletion of nef that was recovered 20 years after primary infection: large pool of proviruses with deletions of env. [J Virol. 2006]
Entire genome of a strain of human immunodeficiency virus type 1 with a deletion of nef that was recovered 20 years after primary infection: large pool of proviruses with deletions of env.
Calugi G, Montella F, Favalli C, Benedetto A. J Virol. 2006 Dec; 80(23):11892-6. Epub 2006 Sep 20.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Nef genes of SIV.
Nef genes of SIV.
J Med Primatol. 1990 ;19(3-4):421-9.

PubMed
Making lemonade out of lemons: a case report and literature review of external p...
Making lemonade out of lemons: a case report and literature review of external pressure as an intervention with pregnant and parenting substance-using women.
J Clin Psychiatry. 2012 Jan ;73(1):51-6.

PubMed
Alstrom's syndrome: further evidence of autosomal recessive inheritance and endo...
Alstrom's syndrome: further evidence of autosomal recessive inheritance and endocrinological dysfunction.
J Med Genet. 1990 Sep ;27(9):590-2.

PubMed
Evaluating the cost effectiveness of donepezil in the treatment of Alzheimer's d...
Evaluating the cost effectiveness of donepezil in the treatment of Alzheimer's disease in Germany using discrete event simulation.
BMC Neurol. 2012 Feb 8 ;12:2.

PubMed
Multilayered carbon nanotube/polymer composite based thermoelectric fabrics.
Multilayered carbon nanotube/polymer composite based thermoelectric fabrics.
Nano Lett. 2012 Mar 14 ;12(3):1307-10. Epub 2012 Feb 16 .

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: