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Proc Natl Acad Sci U S A. 2012 Feb 21;109(8):2919-24. doi: 10.1073/pnas.1111662109. Epub 2012 Feb 6.

Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates.

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  • 1Department of Engineering Science, University of Oxford, Parks Road, Oxford OX1 3PJ, United Kingdom.

Abstract

Multiphoton microscopy is a powerful tool in neuroscience, promising to deliver important data on the spatiotemporal activity within individual neurons as well as in networks of neurons. A major limitation of current technologies is the relatively slow scan rates along the z direction compared to the kHz rates obtainable in the x and y directions. Here, we describe a custom-built microscope system based on an architecture that allows kHz scan rates over hundreds of microns in all three dimensions without introducing aberration. We further demonstrate how this high-speed 3D multiphoton imaging system can be used to study neuronal activity at millisecond resolution at the subcellular as well as the population level.

PMID:
22315405
[PubMed - indexed for MEDLINE]
PMCID:
PMC3286923
Free PMC Article
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