Multifluorescence 2D gel imaging and image analysis

Ingo Vormbrock; Sonja Hartwig; Stefan Lehr

doi:10.1007/978-1-61779-573-2_3

Multifluorescence 2D gel imaging and image analysis

Methods Mol Biol. 2012:854:21-30. doi: 10.1007/978-1-61779-573-2_3.

Authors

Ingo Vormbrock¹, Sonja Hartwig, Stefan Lehr

Affiliation

¹ Institute of Clinical Biochemistry and Pathobiochemistry, German Diabetes Center, Düsseldorf, Germany.

PMID: 22311751
DOI: 10.1007/978-1-61779-573-2_3

Abstract

Although image acquisition and analysis are crucial steps within the multifluorescence two-dimensional gel electrophoresis workflow, some basics are frequently not carried out with the necessary diligence. This chapter should help to prevent easily avoidable failures during imaging and image preparation for comparative protein analysis.

MeSH terms

Image Processing, Computer-Assisted / methods*
Software
Spectrometry, Fluorescence / methods*
Two-Dimensional Difference Gel Electrophoresis / methods*