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Cancer Discov. 2011 Jul;1(2):144-57. doi: 10.1158/2159-8274.CD-11-0010.

Ovarian cancer spheroids use myosin-generated force to clear the mesothelium.

Author information

  • 1Department of Cell Biology, Harvard Medical School, Boston, 02115 MA, USA.

Erratum in

  • Cancer Discov. 2011 Dec;1(7):626. Ince, Tan [corrected to Ince, Tan A].

Abstract

Dissemination of ovarian tumors involves the implantation of cancer spheroids into the mesothelial monolayer on the walls of peritoneal and pleural cavity organs. Biopsies of tumors attached to peritoneal organs show that mesothelial cells are not present under tumor masses. We have developed a live, image-based in vitro model in which interactions between tumor spheroids and mesothelial cells can be monitored in real time to provide spatial and temporal understanding of mesothelial clearance. Here we provide evidence that ovarian cancer spheroids utilize integrin- and talin- dependent activation of myosin and traction force to promote mesothelial cells displacement from underneath a tumor cell spheroid. These results suggest that ovarian tumor cell clusters gain access to the sub-mesothelial environment by exerting force on the mesothelial cells lining target organs, driving migration and clearance of the mesothelial cells.

KEYWORDS:

force; integrins; mesothelium; myosin; ovarian cancer

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PMID:
22303516
[PubMed - indexed for MEDLINE]
PMCID:
PMC3269166
Free PMC Article
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