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Cold Spring Harb Protoc. 2012 Feb 1;2012(2):162-73. doi: 10.1101/pdb.top067785.

Experimental methods for examining synaptic plasticity in Drosophila.


The Drosophila neuromuscular junction (NMJ) ranks as one of the preeminent model systems for studying synaptic development, function, and plasticity. In this article, we review the experimental genetic methods that include the use of mutated or reengineered ion channels to manipulate the synaptic connections made by motor neurons onto larval body-wall muscles. We also provide a consideration of environmental and rearing conditions that phenocopy some of the genetic manipulations.

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