Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Gene. 2012 Mar 15;496(1):45-8. doi: 10.1016/j.gene.2012.01.021. Epub 2012 Jan 24.

Identification of ORF sequences and exercise-induced expression change in thoroughbred horse OXCT1 gene.

Author information

  • 1Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan 609-735, Republic of Korea.

Erratum in

  • Gene. 2012 Aug 10;504(2):318.

Abstract

In the mitochondrial matrix, the OXCT1 gene catalyzes the reversible transfer of coenzyme A from succinyl-CoA to acetoacetate in a reaction related to energy production from ketone bodies. Here, horse OXCT1 gene containing coenzyme A transferase domain was identified in the transcriptome analysis of cDNAs derived from skeletal muscles. Horse OXCT1 gene consisted of 1761 [corrected] nucleotide sequences with an open reading frame of 1560 nucleotides encoding a protein of 520 putative amino acid residues.The number of non-synonymous substitutions was lower than the number of synonymous substitutions in the OXCT1 genes of other species, indicating that purifying selection occurred in the OXCT1 genes during evolutionary radiation. Quantitative real-time RT-RCR analysis showed a dominant expression pattern of horse OXCT1 gene in the cerebrum, heart, and skeletal muscle. Different expression levels of horse OXCT1 transcripts between before- and after-exercise samples were also measured in the skeletal muscles of six horses. These data could be of great use for further investigation of the relationship between energy products and horse OXCT1 gene.

Copyright © 2012. Published by Elsevier B.V.

PMID:
22301269
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk