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Opt Lett. 2012 Feb 1;37(3):431-3. doi: 10.1364/OL.37.000431.

Stimulated Raman hyperspectral imaging based on spectral filtering of broadband fiber laser pulses.

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  • 1Department of Material and Life Science, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan.‑


We demonstrate a technique of hyperspectral imaging in stimulated Raman scattering (SRS) microscopy using a tunable optical filter, whose transmission wavelength can be varied quickly by a galvanometer mirror. Experimentally, broadband Yb fiber laser pulses are synchronized with picosecond Ti:sapphire pulses, and then spectrally filtered out by the filter. After amplification by fiber amplifiers, we obtain narrowband pulses with a spectral width of <3.3 cm(-1) and a wavelength tunability of >225 cm(-1). By using these pulses, we accomplish SRS imaging of polymer beads with spectral information.

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