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J Biomed Biotechnol. 2012;2012:894708. doi: 10.1155/2012/894708. Epub 2012 Jan 16.

The isolation of a new S-methyl benzothioate compound from a marine-derived Streptomyces sp.

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  • 1Faculty of Food Science and Technology, Universiti Putra Malaysia, Selangor, Serdang, Malaysia. norainy@food.upm.edu.my

Abstract

The application of an HPLC bioactivity profiling/microtiter plate technique in conjunction with microprobe NMR instrumentation and access to the AntiMarin database has led to the isolation of a new 1. In this example, 1 was isolated from a cytotoxic fraction of an extract obtained from marine-derived Streptomyces sp. cultured on Starch Casein Agar (SCA) medium. The 1D and 2D (1)H NMR and ESIMS data obtained from 20 μg of compound 1 fully defined the structure. The known 2 was also isolated and readily dereplicated using this approach.

PMID:
22291452
[PubMed - indexed for MEDLINE]
PMCID:
PMC3265089
Free PMC Article
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