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Nat Methods. 2012 Jan 29;9(3):263-5. doi: 10.1038/nmeth.1867.

Lipidic phase membrane protein serial femtosecond crystallography.

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  • 1Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.

Abstract

X-ray free electron laser (X-FEL)-based serial femtosecond crystallography is an emerging method with potential to rapidly advance the challenging field of membrane protein structural biology. Here we recorded interpretable diffraction data from micrometer-sized lipidic sponge phase crystals of the Blastochloris viridis photosynthetic reaction center delivered into an X-FEL beam using a sponge phase micro-jet.

PMID:
22286383
[PubMed - indexed for MEDLINE]
PMCID:
PMC3438231
Free PMC Article

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