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Mol Cell. 2012 Feb 10;45(3):422-32. doi: 10.1016/j.molcel.2011.11.028. Epub 2012 Jan 26.

Extensive DNA damage-induced sumoylation contributes to replication and repair and acts in addition to the mec1 checkpoint.

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  • 1Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10065, USA.

Abstract

The cellular response to DNA damage employs multiple dynamic protein modifications to exert rapid and adaptable effects. Substantial work has detailed the roles of canonical checkpoint-mediated phosphorylation in this program. Recent studies have also implicated sumoylation in the DNA damage response; however, a systematic view of the contribution of sumoylation to replication and repair and its interplay with checkpoints is lacking. Here, using a biochemical screen in yeast, we establish that DNA damage-induced sumoylation occurs on a large scale. We identify MRX (Mre11-Rad50-Xrs2) as a positive regulator of this induction for a subset of repair targets. In addition, we find that defective sumoylation results in failure to complete replication of a damaged genome and impaired DNA end processing, highlighting the importance of the SUMO-mediated response in genome integrity. We also show that DNA damage-induced sumoylation does not require Mec1 checkpoint signaling, and the presence of both enables optimal DNA damage resistance.

Copyright © 2012 Elsevier Inc. All rights reserved.

PMID:
22285753
[PubMed - indexed for MEDLINE]
PMCID:
PMC3340930
Free PMC Article

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