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J Clin Microbiol. 2012 Apr;50(4):1475-7. doi: 10.1128/JCM.00664-11. Epub 2012 Jan 18.

Evaluation of adding a second marker to overcome Staphylococcus aureus spa typing homoplasies.

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  • 1Hospital Preventive Medicine Service, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Lausanne, Switzerland.


The utility of sequencing a second highly variable locus in addition to the spa gene (e.g., double-locus sequence typing [DLST]) was investigated to overcome limitations of a Staphylococcus aureus single-locus typing method. Although adding a second locus seemed to increase discriminatory power, it was not sufficient to definitively infer evolutionary relationships within a single multilocus sequence type (ST-5).

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