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PLoS One. 2012;7(1):e29914. doi: 10.1371/journal.pone.0029914. Epub 2012 Jan 11.

Persistence of Borrelia burgdorferi in rhesus macaques following antibiotic treatment of disseminated infection.

Author information

  • 1Divisions of Bacteriology & Parasitology, Tulane National Primate Research Center, Tulane University Health Sciences Center, Covington, Louisiana, United States of America. members@tulane.edu

Erratum in

  • PLoS One. 2012;7(4):10.1371/annotation/4cafed66-fb84-4589-a001-131d9c50aea6.
  • PLoS One. 2013;8(9). doi:10.1371/annotation/f84663e3-0a2c-4243-8f97-3a58133c1b0f.

Abstract

The persistence of symptoms in Lyme disease patients following antibiotic therapy, and their causes, continue to be a matter of intense controversy. The studies presented here explore antibiotic efficacy using nonhuman primates. Rhesus macaques were infected with B. burgdorferi and a portion received aggressive antibiotic therapy 4-6 months later. Multiple methods were utilized for detection of residual organisms, including the feeding of lab-reared ticks on monkeys (xenodiagnosis), culture, immunofluorescence and PCR. Antibody responses to the B. burgdorferi-specific C6 diagnostic peptide were measured longitudinally and declined in all treated animals. B. burgdorferi antigen, DNA and RNA were detected in the tissues of treated animals. Finally, small numbers of intact spirochetes were recovered by xenodiagnosis from treated monkeys. These results demonstrate that B. burgdorferi can withstand antibiotic treatment, administered post-dissemination, in a primate host. Though B. burgdorferi is not known to possess resistance mechanisms and is susceptible to the standard antibiotics (doxycycline, ceftriaxone) in vitro, it appears to become tolerant post-dissemination in the primate host. This finding raises important questions about the pathogenicity of antibiotic-tolerant persisters and whether or not they can contribute to symptoms post-treatment.

PMID:
22253822
[PubMed - indexed for MEDLINE]
PMCID:
PMC3256191
Free PMC Article
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