Display Settings:

Format

Send to:

Choose Destination
We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Mol Ther. 2012 Mar;20(3):616-24. doi: 10.1038/mt.2011.277. Epub 2012 Jan 10.

A general RNA motif for cellular transfection.

Author information

  • 1Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461, USA.

Abstract

We have developed a selection scheme to generate nucleic acid sequences that recognize and directly internalize into mammalian cells without the aid of conventional delivery methods. To demonstrate the generality of the technology, two independent selections with different starting pools were performed against distinct target cells. Each selection yielded a single highly functional sequence, both of which folded into a common core structure. This internalization signal can be adapted for use as a general purpose reagent for transfection into a wide variety of cell types including primary cells.

Comment in

PMID:
22233578
[PubMed - indexed for MEDLINE]
PMCID:
PMC3294222
Free PMC Article

Images from this publication.See all images (6)Free text

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group Icon for PubMed Central
    Loading ...
    Write to the Help Desk