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Methods Cell Biol. 2012;107:151-75. doi: 10.1016/B978-0-12-394620-1.00005-9.

Culture and manipulation of embryonic cells.

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  • 1Department of Molecular, Cellular, and Developmental Biology, University of Colorado Boulder, Colorado, USA.

Abstract

The direct manipulation of embryonic cells is an important tool for addressing key questions in cell and developmental biology. C. elegans is relatively unique among genetic model systems in being amenable to manipulation of embryonic cells. Embryonic cell manipulation has allowed the identification of cell interactions by direct means, and it has been an important technique for dissecting mechanisms by which cell fates are specified, cell divisions are oriented, and morphogenesis is accomplished. Here, we present detailed methods for isolating, manipulating and culturing embryonic cells of C. elegans.

Copyright © 2011 Elsevier Inc. All rights reserved.

PMID:
22226523
[PubMed - indexed for MEDLINE]
PMCID:
PMC3319665
Free PMC Article
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