Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Cell. 2012 Jan 20;148(1-2):285-95. doi: 10.1016/j.cell.2011.12.007. Epub 2012 Jan 5.

Generation of chimeric rhesus monkeys.

Author information

  • 1Oregon National Primate Research Center, Oregon Health & Science University, 505 N.W. 185(th) Avenue, Beaverton, OR 97006, USA.

Abstract

Totipotent cells in early embryos are progenitors of all stem cells and are capable of developing into a whole organism, including extraembryonic tissues such as placenta. Pluripotent cells in the inner cell mass (ICM) are the descendants of totipotent cells and can differentiate into any cell type of a body except extraembryonic tissues. The ability to contribute to chimeric animals upon reintroduction into host embryos is the key feature of murine totipotent and pluripotent cells. Here, we demonstrate that rhesus monkey embryonic stem cells (ESCs) and isolated ICMs fail to incorporate into host embryos and develop into chimeras. However, chimeric offspring were produced following aggregation of totipotent cells of the four-cell embryos. These results provide insights into the species-specific nature of primate embryos and suggest that a chimera assay using pluripotent cells may not be feasible.

Copyright © 2012 Elsevier Inc. All rights reserved.

Comment in

PMID:
22225614
[PubMed - indexed for MEDLINE]
PMCID:
PMC3264685
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for PubMed Central
    Loading ...
    Write to the Help Desk