Plasmid. 2011 Dec 26. [Epub ahead of print]

Development of a genetic tool for activating chromosomal expression of cryptic or tightly regulated loci in Pseudomonas aeruginosa.

Spagnolo J, Bigot S, Denis Y, Bordi C, de Bentzmann S.

Source

Laboratoire d'Ingénierie des Systèmes Macromoléculaires, CNRS - Aix Marseille Université, 31 Chemin Joseph Aiguier, 13402 Marseille, France.

Abstract

A method for replacing endogenous promoter by a constitutive promoter in Pseudomonas aeruginosa is described. Plasmid pKNG101, a broadly used shuttle suicide vector in P. aeruginosa, was improved to allow chromosomal introduction of a Plac promoter in front of any kind of gene especially those with unknown function. Using this strategy alleviates the need for cloning difficulties encountered in this bacteria and antibiotic marker selection.

PMID:: 22212534; [PubMed - as supplied by publisher]

LinkOut - more resources

Full Text Sources

Supplemental Content

Related citations

Shuttle vector for Escherichia coli, Pseudomonas putida, and Pseudomonas aeruginosa. [Basic Life Sci. 1985]
Shuttle vector for Escherichia coli, Pseudomonas putida, and Pseudomonas aeruginosa.
Lushnikov AA, Andreevskaya EA, Lukashova LI, Lobach JB, Domaradskij IV. Basic Life Sci. 1985; 30:657-62.
An improved method for rapid generation of unmarked Pseudomonas aeruginosa deletion mutants. [BMC Microbiol. 2005]
An improved method for rapid generation of unmarked Pseudomonas aeruginosa deletion mutants.
Choi KH, Schweizer HP. BMC Microbiol. 2005 May 23; 5:30. Epub 2005 May 23.
Development of tools for the genetic manipulation of Pseudomonas aeruginosa. [J Microbiol Methods. 2004]
Development of tools for the genetic manipulation of Pseudomonas aeruginosa.
Suh SJ, Silo-Suh LA, Ohman DE. J Microbiol Methods. 2004 Aug; 58(2):203-12.
Integration-proficient plasmids for Pseudomonas aeruginosa: site-specific integration and use for engineering of reporter and expression strains. [Plasmid. 2000]
Integration-proficient plasmids for Pseudomonas aeruginosa: site-specific integration and use for engineering of reporter and expression strains.
Hoang TT, Kutchma AJ, Becher A, Schweizer HP. Plasmid. 2000 Jan; 43(1):59-72.
Review Pseudomonas aeruginosa chromosomal beta-lactamase in patients with cystic fibrosis and chronic lung infection. Mechanism of antibiotic resistance and target of the humoral immune response. [APMIS Suppl. 2003]
Review Pseudomonas aeruginosa chromosomal beta-lactamase in patients with cystic fibrosis and chronic lung infection. Mechanism of antibiotic resistance and target of the humoral immune response.
Ciofu O. APMIS Suppl. 2003; (116):1-47.

See reviews... See all...

Recent activity

Clear Turn Off Turn On

Development of a genetic tool for activating chromosomal expression of cryptic o...
Development of a genetic tool for activating chromosomal expression of cryptic or tightly regulated loci in Pseudomonas aeruginosa.
Plasmid. 2011 Dec 26. [Epub ahead of print]

PubMed
Structural and mechanistic insights into unusual thiol disulfide oxidoreductase.
Structural and mechanistic insights into unusual thiol disulfide oxidoreductase.
J Biol Chem. 2012 Jan 13 ;287(3):1688-97. Epub 2011 Nov 28 .

PubMed
Bacterial interactomes: from interactions to networks.
Bacterial interactomes: from interactions to networks.
Methods Mol Biol. 2012 ;804:15-33.

PubMed
Inside the complex regulation of Pseudomonas aeruginosa chaperone usher systems.
Inside the complex regulation of Pseudomonas aeruginosa chaperone usher systems.
Environ Microbiol. 2011 Dec 22. doi: 10.1111/j.1462-2920.2011.02673.x. [Epub ahead of print]

PubMed
Towards a structural comprehension of bacterial type VI secretion systems: chara...
Towards a structural comprehension of bacterial type VI secretion systems: characterization of the TssJ-TssM complex of an Escherichia coli pathovar.
PLoS Pathog. 2011 Nov ;7(11):e1002386. Epub 2011 Nov 10 .

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Display Settings:

Send to:

Development of a genetic tool for activating chromosomal expression of cryptic or tightly regulated loci in Pseudomonas aeruginosa.

Source

Abstract

LinkOut - more resources

Full Text Sources

Supplemental Content

Related citations

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information