Send to:

Choose Destination
See comment in PubMed Commons below
Plasmid. 2012 May;67(3):245-51. doi: 10.1016/j.plasmid.2011.12.006. Epub 2011 Dec 26.

Development of a genetic tool for activating chromosomal expression of cryptic or tightly regulated loci in Pseudomonas aeruginosa.

Author information

  • 1Laboratoire d'Ingénierie des Systèmes Macromoléculaires, CNRS-Aix Marseille Université, Marseille, France.


A method for replacing endogenous promoter by a constitutive promoter in Pseudomonas aeruginosa is described. Plasmid pKNG101, a broadly used shuttle suicide vector in P. aeruginosa, was improved to allow chromosomal introduction of a Plac promoter in front of any kind of gene especially those with unknown function. Using this strategy alleviates the need for cloning difficulties encountered in this bacteria and antibiotic marker selection.

Copyright © 2011 Elsevier Inc. All rights reserved.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk