A - Apoptosis induced by AcD in MEC. (a) Caspase 3/7 activation and (b) PS externalization in MEC exposed to AcD (100 ng/ml). Data are means ± S.E., n=10, *p<0.05 vs control. B - AcD-induced oxidation of CL in MEC. (a) Typical negative mode ESI-MS spectrum of CL obtained from MEC, LC/MS quantitative assessment of oxidizable (b) and oxidized (c) CL molecular species. CL oxidation products with 1–4 oxygens in each oxidized CL molecular species were detected and shown on inserts. (d) Base peak chromatogram of CL molecular species with m/z 1464. A higher intensity of the peak with m/z 1464 corresponding to oxygenated CL molecular species ((C_18:2)₃(C_18:2-OH), retention time 12.2 min) was detected in AcD treated MEC. Data are means ± S.E., n=7, *p<0.03 vs control.

A – LC/MS base peak profiles of TPP-_C18:1 and TPP in mitochondria from MEC exposed to TPP-_C18:1. B - Quantitative assessment of TPP-_C18:1 and TPP in mitochondria. Data are means ± S.E. n=6.

A- Effect of TPP-C_18:1 on the content of CL molecular species in MEC. Content of CL molecular species in control (a) and TPP-C_18:1 treated cells (b). Content of C_18:1 (c) and changes in CL fatty acid composition (d) in MEC treated with TPP-C_18:1. The amounts of CL molecular species with m/z 1448, m/z 1472, m/z 1494 and m/z 1496 in TPP-C_18:1 treated cells were dropped to 21.8 ± 3.3, 7.2 ± 2.0, 6.5 ± 2.3 and 5.4 ± 1.8 pmol/nmol of total CL from 36.5 ± 3.6, 17.9 ± 4.3, 8.7 ± 1.6 and 8.6 ± 1.4 pmol/nmol of total CL in control cells, respectively. Consequently, the amount of CL molecular species with m/z 1456 was increased from 10.8 ± 2.7 to 58.6 ± 7.2 pmol/nmol of total CL. Data are means ± S.E., n = 3–10. B - Effect of TPP-C_18:1 on AcD-induced phosphatidylserine externalization (a) and caspase 3/7 activation (b) in MEC. Data are means ± S.E., n=8, *p<0.05 vs control.

A -Effect of AcD, TPP-C_18:1 and triacsin C on CL oxidation in MEC. Data are presented as increase of oxidized CL molecular species. Data are means ± S.E., n=3–7, *p<0.05 vs control. B - Effect of TPP-C_18:1 and triacsin C on AcD-induced phopsphatidylserine expernalization (a) and caspase 3/7 activation (b) in MEC. Data are means ± S.E., n=3–10, *p<0.05 vs control. C- Content of CL molecular species in triacsin C treated (a) and triacsin C treated TPP-C_18:1 exposed cells (b). The amounts of CL molecular species with m/z 1448, m/z 1472, m/z 1494 and m/z 1496 were estimated as 33.3 ± 1.9, 13.8 ± 2.0, 9.6 ± 1.8 and 6.5 ± 1.6 pmol/nmol of total CL in triacsin C pre-treated and 37.6 ± 3.9, 11.0 ± 3.1, 6.6 ± 4.3 and 3.9 ± 3.1 pmol/nmol of total CL in triacsin pre-treated/TPP-C_18:1 exposed cells, respectively. Consequently, the amount of CL molecular species with m/z 1456 was 16.4 ± 3.4 to 24.7 ± 4.2 pmol/nmol of total CL. Data are means ± S.E., n=3–5.