Display Settings:

Format

Send to:

Choose Destination
PLoS One. 2011;6(12):e28998. doi: 10.1371/journal.pone.0028998. Epub 2011 Dec 20.

APC/C(Cdh1)-mediated degradation of the F-box protein NIPA is regulated by its association with Skp1.

Author information

  • 1Department of Internal Medicine ΙΙΙ, Technical University of Munich, Munich, Germany.

Abstract

NIPA (Nuclear Interaction Partner of Alk kinase) is an F-box like protein that targets nuclear Cyclin B1 for degradation. Integrity and therefore activity of the SCF(NIPA) E3 ligase is regulated by cell-cycle-dependent phosphorylation of NIPA, restricting substrate ubiquitination to interphase. Here we show that phosphorylated NIPA is degraded in late mitosis in an APC/C(Cdh1)-dependent manner. Binding of the unphosphorylated form of NIPA to Skp1 interferes with binding to the APC/C-adaptor protein Cdh1 and therefore protects unphosphorylated NIPA from degradation in interphase. Our data thus define a novel mode of regulating APC/C-mediated ubiquitination.

PMID:
22205987
[PubMed - indexed for MEDLINE]
PMCID:
PMC3243670
Free PMC Article

Images from this publication.See all images (4)Free text

Figure 1
Figure 2
Figure 3
Figure 4
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for Public Library of Science Icon for PubMed Central
    Loading ...
    Write to the Help Desk