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PLoS One. 2011;6(12):e28954. doi: 10.1371/journal.pone.0028954. Epub 2011 Dec 16.

Quantitative field testing Rotylenchulus reniformis DNA from metagenomic samples isolated directly from soil.

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  • 1Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Mississippi State, Mississippi, United States of America.


A quantitative PCR procedure targeting the β-tubulin gene determined the number of Rotylenchulus reniformis Linford & Oliveira 1940 in metagenomic DNA samples isolated from soil. Of note, this outcome was in the presence of other soil-dwelling plant parasitic nematodes including its sister genus Helicotylenchus Steiner, 1945. The methodology provides a framework for molecular diagnostics of nematodes from metagenomic DNA isolated directly from soil.

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