Send to:

Choose Destination
See comment in PubMed Commons below
J Clin Microbiol. 2012 Mar;50(3):1057-60. doi: 10.1128/JCM.05987-11. Epub 2011 Dec 14.

Engineered combined-positive-control template for real-time reverse transcription-PCR in multiple-pathogen-detection assays.

Author information

  • 1Division of Bacterial Diseases, Respiratory Diseases Branch, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention,Atlanta, Georgia, USA.


Recently we evaluated a custom TaqMan array card (TAC) detection system, formerly known as a TaqMan low-density array (TLDA) card, for simultaneous real-time PCR identification of 21 pathogens and three control targets in duplicate from respiratory specimens (M. Kodani et al., J. Clin. Microbiol. 49:2175-2182, 2011). We engineered an adaptable and expandable system of in vitro RNA transcripts to serve as a combined positive control for both DNA and RNA targets in multiple-pathogen-detection technologies based on real-time reverse transcription-PCR.

[PubMed - indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk