Display Settings:

Format

Send to:

Choose Destination
J Clin Microbiol. 2012 Mar;50(3):1057-60. doi: 10.1128/JCM.05987-11. Epub 2011 Dec 14.

Engineered combined-positive-control template for real-time reverse transcription-PCR in multiple-pathogen-detection assays.

Author information

  • 1Division of Bacterial Diseases, Respiratory Diseases Branch, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention,Atlanta, Georgia, USA.

Abstract

Recently we evaluated a custom TaqMan array card (TAC) detection system, formerly known as a TaqMan low-density array (TLDA) card, for simultaneous real-time PCR identification of 21 pathogens and three control targets in duplicate from respiratory specimens (M. Kodani et al., J. Clin. Microbiol. 49:2175-2182, 2011). We engineered an adaptable and expandable system of in vitro RNA transcripts to serve as a combined positive control for both DNA and RNA targets in multiple-pathogen-detection technologies based on real-time reverse transcription-PCR.

PMID:
22170926
[PubMed - indexed for MEDLINE]
PMCID:
PMC3295119
Free PMC Article

Images from this publication.See all images (1)Free text

Fig 1
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk