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Methods Mol Biol. 2012;831:359-68. doi: 10.1007/978-1-61779-480-3_19.

Assignment of backbone resonances in a eukaryotic protein kinase - ERK2 as a representative example.

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  • 1Department of Chemistry, The City College of New York, New York, NY, USA.

Abstract

A first step toward the analysis of the structure, dynamics, and interactions of proteins by NMR is obtaining an acceptable level of resonance assignments. This process is nontrivial in most eukaryotic kinases given their size and suboptimal behavior in solution. Using inactive ERK2 as a representative example, we describe the procedures we utilized to achieve a significant degree of completeness of backbone resonance assignment.

PMID:
22167683
[PubMed - indexed for MEDLINE]
PMCID:
PMC3305997
Free PMC Article

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