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    Sensors (Basel). 2011;11(6):5740-53. Epub 2011 May 27.

    Effects of Al(III) and nano-Al13 species on malate dehydrogenase activity.

    Yang X, Cai L, Peng Y, Li H, Chen RF, Shen RF.

    Source

    Jiangsu Key Laboratory of Biofunctional Materials, Laboratory of Electrochemistry, College of Chemistry and Material Science, Nanjing Normal University, Nanjing, China. yangxiaodi@njnu.edu.cn

    Abstract

    The effects of different aluminum species on malate dehydrogenase (MDH) activity were investigated by monitoring amperometric i-t curves for the oxidation of NADH at low overpotential using a functionalized multi-wall nanotube (MWNT) modified glass carbon electrode (GCE). The results showed that Al(III) and Al(13) can activate the enzymatic activity of MDH, and the activation reaches maximum levels as the Al(III) and Al(13) concentration increase. Our study also found that the effects of Al(III) and Al(13) on the activity of MDH depended on the pH value and aluminum speciation. Electrochemical and circular dichroism spectra methods were applied to study the effects of nano-sized aluminum compounds on biomolecules.

    PMID:
    22163924
    [PubMed - in process]
    PMCID:
    PMC3231464
    Free PMC Article

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