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J Clin Microbiol. 2012 Mar;50(3):798-809. doi: 10.1128/JCM.05733-11. Epub 2011 Dec 7.

Comparison of molecular typing methods useful for detecting clusters of Campylobacter jejuni and C. coli isolates through routine surveillance.

Author information

  • 1National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada. Clifford.Clark@phac-aspc.gc.ca

Abstract

Campylobacter spp. may be responsible for unreported outbreaks of food-borne disease. The detection of these outbreaks is made more difficult by the fact that appropriate methods for detecting clusters of Campylobacter have not been well defined. We have compared the characteristics of five molecular typing methods on Campylobacter jejuni and C. coli isolates obtained from human and nonhuman sources during sentinel site surveillance during a 3-year period. Comparative genomic fingerprinting (CGF) appears to be one of the optimal methods for the detection of clusters of cases, and it could be supplemented by the sequencing of the flaA gene short variable region (flaA SVR sequence typing), with or without subsequent multilocus sequence typing (MLST). Different methods may be optimal for uncovering different aspects of source attribution. Finally, the use of several different molecular typing or analysis methods for comparing individuals within a population reveals much more about that population than a single method. Similarly, comparing several different typing methods reveals a great deal about differences in how the methods group individuals within the population.

PMID:
22162562
[PubMed - indexed for MEDLINE]
PMCID:
PMC3295161
Free PMC Article

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