The aim of this study was to analyze surface electromyography activity (sEMG) and rating of perceived exertion (RPE) responses in different muscles while standing on a vibrating platform producing oscillations of different frequencies and amplitudes. Twenty community-dwelling older adults (79.6 ± 3.2 years) took part in the research. Subjects were exposed to 12 different vibration treatments of 15 seconds separated by 1 minute of rest in random order to check the influence of frequency (25, 35, and 45 Hz) and amplitude (1 mm [low] and 3.1 mm [high]) vibration on sEMG signal and RPE. Additionally, the use of a soft pad was also examined for its influence on these measures. Three-factor analysis of variance for RPE and both lower and whole-body sEMGs revealed a significant amplitude main effect (p < 0.01), and soft mat effect (p < 0.01), and a significant frequency main effect (p < 0.01). The major findings were that sEMG and RPE increased with the acceleration of the vibration; moreover, the increments of sEMG were highly correlated with RPE. The results of this study suggest that using the RPE method after each exercise would allow exercise and health professionals to assess the intensity levels that correspond to the level of the vibratory program in older adults.