Format

Send to

Choose Destination
See comment in PubMed Commons below
Methods Mol Biol. 2012;814:545-54. doi: 10.1007/978-1-61779-452-0_36.

In vivo imaging of Ca²⁺ signaling in astrocytes using two-photon laser scanning fluorescent microscopy.

Author information

  • 1Department of Biological Engineering, Dalton Cardiovascular Research Center, University of Missouri-Columbia, Columbia, MO, USA. dings@missouri.edu

Abstract

Astrocytes are the predominant nonneuronal cell type in the central nervous system. Although they are electrically nonexcitable, they have been found to play an active role in modulation of neuronal function and plasticity through Ca(2+) excitability. Thus, Ca(2+) signaling in astrocytes serves as a mediator of bidirectional interactions between neurons and astrocytes. Although astrocytic Ca(2+) signaling has been extensively studied in cultured cells, the recent development of two-photon laser scanning fluorescent microscopy and astrocyte-specific dye labeling make it possible to study astrocytic Ca(2+) signaling in live animals. Here we describe a detailed protocol for in vivo Ca(2+) imaging of astrocytes in mice.

[PubMed - indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Springer Icon for PubMed Central
    Loading ...
    Write to the Help Desk